STUDY OF PHENOTYPIC CHARACTERISTICS AND THE EFFECT OF SOME ANTIFUNGALS AGAINST THE FUNGUS TRICHOPHYTON MENTAGROPHYTES THAT CAUSES TINEA CORPORIS IN KIRKUK CITY

Abstract

This study was conducted in the graduate studies laboratory in the College of Science at the University of Kirkuk for the duration 7/11/2021 to 5/25/2022 with the goal of investigating dermatomycosis fungi in the human body (tinea corporis) and the incidence of Trichophyton mentagrophytes. a hundred and twenty samples were accrued from sufferers attending a dermatology consultation at Azadi Teaching Hospital and some personal clinics in the city of Kirkuk between (1 - 60)age groups and for each genders. The samples included taken from areas of the pores and skin in quite a number parts of the physique and under the direct supervision of the professional doctor. Skin samples were taken scraping method, The affected area was sterilized with ethyl alcohol at a awareness of 70%, and then the scales were scraped from the side of the fungus-infected focal point using a sharp sterilized blade, and the samples were recognized by means of direct microscopy the use of koh at a attention of 10%, and the closing part of the pathological samples was once cultivated) on dishes containing saproid dextrose agar (SDA) medium to which cycloheximide was added. To forestall the increase of saprophytic fungi and chloramphenicol to forestall the growth of bacteria, the occlusion was checked continuously to note fungal growth. Direct microscopic examination of fungi confirmed wonderful results, with an contamination fee of 66.66% of the complete one hundred twenty samples, whilst laboratory subculture confirmed high quality results, with a rate of 49.16% of the total of one hundred twenty samples. The results of the phenotypic examinations of the remoted dermatophytes confirmed (they belong to both genes, Trichophyton and Microsporum). All samples were recognized by using common techniques the usage of unique way of life media and based on the phenotypic characteristics of the colonies in addition to the microscopic characteristics. The outcomes of the current study showed that T.mentagrophytes is greater frequent among patients suffering from tinea corporis than other types of skin fungi, with a rate of (9.2%). And the percentage of infection with skin fungi differed according to the region of residence, as it used to be extra common in the rural area (62.5%) and the lowest number of infections in the town (37.5%). A study used to be conducted of the effect of antifungals nystatin, amphotricin B, ketoconazole, fluconazole and clotrimazole in opposition to T.mentagrophytes, as the effects confirmed that the antifungal ketoconazole had higher inhibition on T.mentagrophytes compared to different antifungals and the least antifungal nystatin inhibited the fungus by means of a small percentage.